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Dr. Bustamante begins his talk by explaining why one would wish to study biochemical reactions at the level of a single molecule. He explains that many processes within the cell are carried out by very few molecules. By studying single molecules, it is possible to obtain details about the mechanism of a reaction that cannot be ascertained by studying a population of molecules. Bustamante goes on to describe the technique of optical tweezers and how it can be used to manipulate single molecules. His lab has successfully used this method to follow DNA transcription one molecule at a time and RNA translation one codon at a time. In both cases, single molecule studies provided detailed information about complex biochemical processes.
This course is intended for the student interested in understanding and appreciating common biological topics in the study of the smallest units within biology: molecules and cells. Molecular and cellular biology is a dynamic field. There are thousands of opportunities within the medical, pharmaceutical, agricultural, and industrial fields (just to name a few) for a person with a concentrated knowledge of molecular and cellular processes. This course will give you a general introduction to these topics. In addition to preparing for a diversity of career paths, an understanding of molecular and cell biology will help you make sound decisions in your everyday life that can positively impact your diet and health. This course includes the following units: Unit 1: Introduction to Biology Unit 2: Basic Chemistry Unit 3: Biological Molecules Unit 4: Cells and Cell Membranes Unit 5: Enzymes, Metabolism, Cellular Respiration Unit 6: Photosynthesis Unit 7: Cellular Reproduction: Mitosis Unit 8: Cellular Reproduction: Meiosis Unit 9: Mendelian Genetics and Chromosomes Unit 10: Gene Expression
A grasp of the logic and practice of science is essential to understand the rest of the world around us. To that end, the CMB3e iText (like earlier editions) remains focused on experimental support for what we know about cell and molecular biology, and on showing students the relationship of cell structure and function. Rather than trying to be a comprehensive reference book, CMB3e selectively details investigative questions, methods and experiments that lead to our understanding of cell biology. This focus is nowhere more obvious than in the chapter learning objectives and in external links to supplementary material. The Basic CMB3e version of the iText includes links to external web-sources as well as the author’s short, just-in-time YouTube VOPs (with edited, optional closed captions), all embedded in or near relevant text. Each video is identified with a descriptive title and video play and QR bar codes. The Learning objectives align with content and ask students to use new knowledge to make connections and deepen their understanding of concept and experiment. All external links are intended to expand or explain textual content and concepts and to engage student curiosity. Links to full VOP lectures are now at the back of the book. include optional edited closed captions. All images in the iText are by the author or are from public domain or Creative Commons (CC) licensed sources. For all externally sourced images, CC licenses are indicated with the image. Beyond the Basic CMB3e, the freely available Annotated CMB3e contains interactive links and formative assessments in the form of Challenge boxes. A CMB3e Sample Chapter and CMB3e iText for Instructors model additional interactive features, including short 25 Words or Less writing assignments that can be incorporated into almost any course management system, and all of which the author assigned as homework in his flipped, blended course. These assessments aim to reinforce writing as well as critical thinking skills. The CMB3e Sample Chapter is freely available for download; the CMB3e for Instructors version of the iText is available on request. My goal in writing this iText is to make the content engaging, free and comparable in accuracy and currency to commercial textbooks. I encourage instructors to use the interactive features of the iText (critical thought questions, YouTube videos, etc.) to challenge their students. With all of these enhancements, I encourage students to think about • how good and great experiments were inspired and designed, • how alternative experimental results were predicted, • how data was interpreted, and finally, • investigators (and we!) arrive at the most interesting “next questions”. The online iText is the most efficient way to access links and complete online assignments. Nevertheless, you can download, read, study, and access many links with a smart phone or tablet. And you can add your own annotations digitally, or write in the margins of a printout the old-fashioned way! Your instructor may provide additional instructions for using your iText.