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Dr. Bustamante begins his talk by explaining why one would wish to study biochemical reactions at the level of a single molecule. He explains that many processes within the cell are carried out by very few molecules. By studying single molecules, it is possible to obtain details about the mechanism of a reaction that cannot be ascertained by studying a population of molecules. Bustamante goes on to describe the technique of optical tweezers and how it can be used to manipulate single molecules. His lab has successfully used this method to follow DNA transcription one molecule at a time and RNA translation one codon at a time. In both cases, single molecule studies provided detailed information about complex biochemical processes.
- Subjects:
- Biochemistry
- Keywords:
- Biomolecules Molecular biology
- Resource Type:
- Video
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Video
Synthetic biology can be used in industrial biotechnology to engineer metabolic pathways to create high-value chemicals using model microorganisms such as yeast. During the Synthetic Biology in Action course, participants engineered yeast to produce beta-caretone for industrial biotechnology purposes. In this talk, they describe the steps they took to engineer an existing yeast pathway to produce the new chemical. These steps include modeling the metabolic pathway outputs, DNA design, amplification, and assembly, and analysis of the final result.
- Subjects:
- Electronic and Information Engineering, Biochemistry, and Biology
- Keywords:
- Synthetic biology Biochemistry Yeast fungi -- Biotechnology
- Resource Type:
- Video