Bioinformatics 101: Practical Computational Biology Knowledge Base

Bioinformatics 101 is an educational web directory and practical training platform maintained by the Bioinformatics and System Biology Group at Zhejiang University (ZJU). Designed to lower the barrier to entry for computational biology, the platform provides structured, step-by-step tutorials, command-line guides, and curated software indices for analyzing high-throughput biological data. The platform covers core bioinformatic workflows, including next-generation sequencing (NGS) data processing, RNA-seq differential expression analysis, single-cell RNA sequencing (scRNA-seq), genomic variant calling, molecular phylogenetics, protein structure prediction, and biological network analysis. It emphasizes practical computing skills, providing hands-on instruction in Linux environment management, shell scripting, R programming, Python for data science, and pipeline execution tools (such as Conda, Docker, and Nextflow). By bridging theoretical molecular biology with hands-on computational execution, Bioinformatics 101 serves as an intuitive self-study manual and teaching aid. As a high-value open educational resource, it offers essential instructional modules, code templates, and analytical workflows for undergraduate and postgraduate students, bench scientists transitioning into dry-lab research, bioinformaticians, and computational biologists studying genomics, systems biology, transcriptomics, and biological data science.